TOLLIP Polyclonal antibody

• Featured Product
• KD/KO Validated

TOLLIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11315-1-AP

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 11315-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11315-1-AP

  WB result of TOLLIP antibody (11315-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.

• 

  WB analysis using 11315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11315-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11315-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11315-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11315-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11315-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11315-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 11315-1-AP

  Immunofluorescent analysis of PC-3 cells using 11315-1-AP (TOLLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, human liver tissue, mouse brain tissue, PC-3 cells, rat brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11315-1-AP targets TOLLIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOLLIP fusion protein Ag1864
• Full Name: toll interacting protein
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012057
• Gene Symbol: TOLLIP
• Gene ID (NCBI): 54472
• RRID: AB_2256373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Publications

Species	Application	Title
human	WB	Autophagy; TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression; Authors - Tai-Mei Zhang
human	WB	PLoS Pathog; The MGF300-2R protein of African swine fever virus is associated with viral pathogenicity by promoting the autophagic degradation of IKKα and IKKβ through the recruitment of TOLLIP; Authors - Tao Wang
pig	WB	J Virol; African Swine Fever Virus L83L Negatively Regulates the cGAS-STING-Mediated IFN-I Pathway by Recruiting Tollip To Promote STING Autophagic Degradation; Authors - Mingyang Cheng
human	WB	mBio; The Globular C1q Receptor Is Required for Epidermal Growth Factor Receptor Signaling during Candida albicans Infection.; Authors - Quynh T Phan ; KD Validated
human	WB	Nat Commun; Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy; Authors - Shouheng Jin ; KD Validated
rat	IF	Gut Microbes; Lactiplantibacillus plantarum 299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats; Authors - Pinar Sargin

Related Products

Rac1 Polyclonal antibody

24072-1-AP

RAC1 Monoclonal antibody

66122-1-Ig

TLR4 Polyclonal antibody

19811-1-AP

IRAK1 Polyclonal antibody

10478-2-AP

TRAF6 Polyclonal antibody

12809-1-AP

IRAK4 Polyclonal antibody

18221-1-AP