TOB1 Recombinant antibody

TOB1 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241043A9

Cat no : 83944-2-RR

Synonyms

Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6

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Validation Data Gallery

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  IF Staining of HeLa using 83944-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOB1 antibody (83944-2-RR, Clone: 241043A9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83944-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-2-RR against Human TOB1 were performed. The affinity constant is 0.462 nM.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83944-2-RR is TOB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOB1 fusion protein Ag6714
• Full Name: transducer of ERBB2, 1
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: BC031406
• Gene Symbol: TOB1
• Gene ID (NCBI): 10140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.