TNS3 Polyclonal antibody

TNS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20053-1-AP

Synonyms

H_NH049I23.2, TEM6, TENS1, tensin 3, TNS3, TPP, Tumor endothelial marker 6

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Validation Data Gallery

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  WB analysis of mouse kidney using 20053-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20053-1-AP (TNS3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 20053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 20053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20053-1-AP targets TNS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tensin 3
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 129 kDa
• GenBank Accession Number: NM_022748
• Gene Symbol: TNS3
• Gene ID (NCBI): 64759
• RRID: AB_10667405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNS3, also named as TEM6, TENS1 and TPP, may play a role in actin remodeling. It is involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. TNS3 seems to be involved in mammary cell migration. It may be involved in cell migration and bone development. The antibody recognizes all the isoforms of TNS3.

Publications

Species	Application	Title
human	WB	Cell Biol Toxicol; EIF4A3-mediated oncogenic circRNA hsa_circ_0001165 advances esophageal squamous cell carcinoma progression through the miR-381-3p/TNS3 pathway; Authors - Xun Zhang