TNS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TNS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20054-1-AP

Synonyms

MST091, MST122, MST127, MSTP122, MSTP127, MXRA6, tensin 1, TNS, TNS1

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Validation Data Gallery

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  WB analysis of mouse heart using 20054-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20054-1-AP (TNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20054-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20054-1-AP (TNS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20054-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20054-1-AP (TNS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 20054-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20054-1-AP (TNS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20054-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20054-1-AP (TNS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20054-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20054-1-AP (TNS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20054-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20054-1-AP (TNS1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

20054-1-AP targets TNS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tensin 1
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 186 kDa
• GenBank Accession Number: NM_022648
• Gene Symbol: TNS1
• Gene ID (NCBI): 7145
• RRID: AB_2878636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNS1, also named as TNS, may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton. The antibody recognizes near the N-term of TNS1.

Publications

Species	Application	Title
human	WB,IHC	Aging (Albany NY); LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis.; Authors - Congyi Tang
human	IF	EBioMedicine; Transcriptomics based multi-dimensional characterization and drug screen in esophageal squamous cell carcinoma.; Authors - Yin Li
human	WB	Biomed Pharmacother; PMID: 31954876; Authors - Xiaogang Sun ; KD Validated
human	IHC	Reproduction; Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.; Authors - Ru Zheng
human	WB	Mutagenesis; TNS1 regulated by HLF represses the progression of prostate cancer; Authors - Hao Zhou
human	WB,IF	Exp Cell Res; MicroRNA-522-3p promotes brain metastasis in non-small cell lung cancer by targeting Tensin 1 and modulating blood-brain barrier permeability; Authors - Qing Liu