TNPO3 Recombinant antibody, PBS Only (Detector)

TNPO3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240728F8

Cat no : 83704-2-PBS

Synonyms

transportin 3, IPO12, Importin-12, Importin 12, Imp12

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Validation Data Gallery

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  WB analysis using 83704-2-RR (same clone as 83704-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83704-2-RR (TNPO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83704-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83704-2-RR (same clone as 83704-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83704-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U-2 OS using 83704-2-RR (same clone as 83704-2-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83704-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00668-1

  Cytometric bead array standard curve of MP00668-1, TNPO3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83704-1-PBS. Detection antibody: 83704-2-PBS. Standard: Ag8769 Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 83704-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83704-2-RR against Human TNPO3 were performed. The affinity constant is below 1 pM.

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Product Information

83704-2-PBS targets TNPO3 as part of a matched antibody pair:

MP00668-1: 83704-1-PBS capture and 83704-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TNPO3 fusion protein Ag8769
• Full Name: transportin 3
• Calculated Molecular Weight: 975 aa, 110 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC009923
• Gene Symbol: TNPO3
• Gene ID (NCBI): 23534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transportin 3 (TNPO3) also known as IPO12, TRNSR, is a karyopherin β that works as a nuclear carrier shuttling, from the cytoplasm to the nucleus, the serine/arginine-rich proteins (PMID: 33452620). TNPO3 specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1, and SFRS2, by recognizing phosphorylated SR domains (PMID: 11517331, 12628928). Mutations in TNPO3 are associated with Autosomal Dominant Limb-Girdle Muscular Dystrophy 2 (PMID: 23543484).