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TNPO3 Recombinant antibody

TNPO3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240728F8

Cat no : 83704-2-RR

Synonyms

transportin 3, IPO12, Importin-12, Importin 12, Imp12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNPO3 Recombinant antibody (83704-2-RR)

    WB analysis using 83704-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83704-2-RR (TNPO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using TNPO3 Recombinant antibody (83704-2-RR)

    FC experiment of HeLa using 83704-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of U-2 OS using TNPO3 Recombinant antibody (83704-2-RR)

    FC experiment of U-2 OS using 83704-2-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83704-2-RR

    Affinity and Kinetic Characterization of 83704-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83704-2-RR against Human TNPO3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, C2C12 cells, NIH/3T3 cells, mouse brain tissue, rat thymus tissue
Positive FC (Intra) detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83704-2-RR is TNPO3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TNPO3 fusion protein Ag8769
Full Name transportin 3
Calculated Molecular Weight 975 aa, 110 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC009923
Gene Symbol TNPO3
Gene ID (NCBI) 23534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transportin 3 (TNPO3) also known as IPO12, TRNSR, is a karyopherin β that works as a nuclear carrier shuttling, from the cytoplasm to the nucleus, the serine/arginine-rich proteins (PMID: 33452620). TNPO3 specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1, and SFRS2, by recognizing phosphorylated SR domains (PMID: 11517331, 12628928). Mutations in TNPO3 are associated with Autosomal Dominant Limb-Girdle Muscular Dystrophy 2 (PMID: 23543484).

Protocols

Product Specific Protocols
WB protocol for TNPO3 antibody 83704-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols