TNK1 Polyclonal antibody

TNK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14199-1-AP

Synonyms

CD38 negative kinase 1, TNK1

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Validation Data Gallery

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  WB analysis using 14199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14199-1-AP targets TNK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNK1 fusion protein Ag5402
• Full Name: tyrosine kinase, non-receptor, 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC035782
• Gene Symbol: TNK1
• Gene ID (NCBI): 8711
• RRID: AB_2205700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Immunol; Thirty-Eight-Negative Kinase 1 Is a Mediator of Acute Kidney Injury in Experimental and Clinical Traumatic Hemorrhagic Shock.; Authors - Rebecca Halbgebauer
human,rat	WB, IHC	3 Biotech; Tyrosine kinase nonreceptor 1 (TNK1) knockdown ameliorates hemorrhage shock-induced kidney injury via inhibiting macrophage M1 polarization.; Authors - Miaolong Tang