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TNIP1 Polyclonal antibody
TNIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15104-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, U2OS cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
IP | See 2 publications below |
Product Information
15104-1-AP targets TNIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TNIP1 fusion protein Ag1561 |
Full Name | TNFAIP3 interacting protein 1 |
Calculated Molecular Weight | 72 kDa |
Observed Molecular Weight | 72 kDa |
GenBank Accession Number | BC014008 |
Gene Symbol | TNIP1 |
Gene ID (NCBI) | 10318 |
RRID | AB_2207757 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs.
Protocols
Product Specific Protocols | |
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WB protocol for TNIP1 antibody 15104-1-AP | Download protocol |
IHC protocol for TNIP1 antibody 15104-1-AP | Download protocol |
IF protocol for TNIP1 antibody 15104-1-AP | Download protocol |
IP protocol for TNIP1 antibody 15104-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 | ||
Cell Death Differ Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development. | ||
BMC Biol A functional genomics screen for microRNA regulators of NF-kappaB signaling.
| ||
Retrovirology Immune regulator ABIN1 suppresses HIV-1 transcription by negatively regulating the ubiquitination of Tat.
| ||
J Cell Biochem ABIN1 inhibits HDAC1 ubiquitination and protects HDAC1 from both proteasome- and lysozyme-dependent degradation.
| ||
Clin Exp Pharmacol Physiol ABIN-1 protects chondrocytes from lipopolysaccharide-induced inflammatory injury through the inactivation of NF-κB signalling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Heiko (Verified Customer) (09-04-2019) | PVDF membrane blocked in 5% milk TBST 1 h at RT.Primary antibody incubation overnight 4°C.
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