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TNIK Recombinant antibody, PBS Only

TNIK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241518D9

Cat no : 84231-4-PBS

Synonyms

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNIK Recombinant antibody (84231-4-RR)

    WB analysis using 84231-4-RR (same clone as 84231-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using TNIK Recombinant antibody (84231-4-RR)

    IF Staining of K-562 using 84231-4-RR (same clone as 84231-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84231-4-RR

    Affinity and Kinetic Characterization of 84231-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

Product Information

The immunogen of 84231-4-PBS is TNIK Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TNIK fusion protein Ag34132
Full Name TRAF2 and NCK interacting kinase
Observed Molecular Weight170 kDa
GenBank Accession NumberBC150256
Gene Symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).