TNIK Polyclonal antibody

TNIK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30906-1-AP

Synonyms

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 30906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 30906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (30906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: COLO 205 cells, HT-29 cells, K-562 cells
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30906-1-AP targets TNIK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNIK fusion protein Ag34132
• Full Name: TRAF2 and NCK interacting kinase
• Observed Molecular Weight: 150~180 kDa
• GenBank Accession Number: BC150256
• Gene Symbol: TNIK
• Gene ID (NCBI): 23043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNIK (Traf2- and Nck-interacting kinase) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension, which had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling (PMID: 24566388). TNIK is also an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462). Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).