TNFRSF9/CD137 Recombinant antibody, PBS Only (Detector)

TNFRSF9/CD137 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241006G5

Cat no : 83893-4-PBS

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00870-2

  Sandwich ELISA standard curve of MP00870-2, Human TNFRSF9/CD137 Recombinant Matched Antibody Pair - PBS only. 83893-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0016. 83893-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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  Sample test of MP00870-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TNFRSF9 concentration was undetermined in unstimulated PBMC supernatant, 173.5 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TNFRSF9/CD137 concentration was undetermined in unstimulated PBMC supernatant, 435.7 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00870-2

  Serum of fifteen individual healthy human donors was measured. The TNFRSF9/CD137 concentration of detected samples was determined to be 360.9 pg/mL with a range of 47.6-1,328.1 pg/mL.

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  Cytometric bead array standard curve of MP00870-2

  Cytometric bead array standard curve of MP00870-2, TNFRSF9/CD137 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83893-1-PBS. Detection antibody: 83893-4-PBS. Standard: Eg0016. Range: 0.313-40 ng/mL.

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Product Information

83893-4-PBS targets TNFRSF9/CD137 as part of a matched antibody pair:

MP00870-2: 83893-1-PBS capture and 83893-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC006196
• Gene Symbol: TNFRSF9/CD137
• Gene ID (NCBI): 3604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)