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TNFRSF9/CD137 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.313-40 ng/mL

Sandwich ELISA: 31.25-2000 pg/mL

Cat No : MP00870-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00870-2, Human TNFRSF9/CD137 Recombinant Matched Antibody Pair - PBS only. 83893-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0016. 83893-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

    Sandwich ELISA standard curve of MP00870-2

    Sandwich ELISA standard curve of MP00870-2, Human TNFRSF9/CD137 Recombinant Matched Antibody Pair - PBS only. 83893-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0016. 83893-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Serum of fifteen individual healthy human donors was measured. The TNFRSF9/CD137 concentration of detected samples was determined to be 360.9 pg/mL with a range of 47.6-1,328.1 pg/mL.

    Sample test of MP00870-2

    Serum of fifteen individual healthy human donors was measured. The TNFRSF9/CD137 concentration of detected samples was determined to be 360.9 pg/mL with a range of 47.6-1,328.1 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TNFRSF9 concentration was undetermined in unstimulated PBMC supernatant, 173.5 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TNFRSF9/CD137 concentration was undetermined in unstimulated PBMC supernatant, 435.7 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP00870-2

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TNFRSF9 concentration was undetermined in unstimulated PBMC supernatant, 173.5 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TNFRSF9/CD137 concentration was undetermined in unstimulated PBMC supernatant, 435.7 pg/mL in PHA stimulated PBMC supernatant.

  • Cytometric bead array standard curve of MP00870-2, TNFRSF9/CD137 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83893-1-PBS. Detection antibody: 83893-4-PBS. Standard: Eg0016. Range: 0.313-40 ng/mL.

    Cytometric bead array standard curve of MP00870-2

    Cytometric bead array standard curve of MP00870-2, TNFRSF9/CD137 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83893-1-PBS. Detection antibody: 83893-4-PBS. Standard: Eg0016. Range: 0.313-40 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00870-2 targets TNFRSF9/CD137 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.313-40 ng/mL (Cytometric Bead Array); 31.25-2000 pg/mL (Sandwich ELISA);

Capture antibody: TNFRSF9 Recombinant antibody, PBS Only (Capture) 83893-1-PBS (241006D2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: TNFRSF9 Recombinant antibody, PBS Only (Detector) 83893-4-PBS (241006G5). 100 μg. Concentration 1 mgl/ml.

Alternative TNFRSF9/CD137 matched antibody pairs: MP00870-1, MP50110-1

Matched pair protein standard: Eg0016

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83893-1-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 9
Synonyms ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor, 4 1BB
GenBank accession numberBC006196
Gene symbol TNFRSF9/CD137
UniprotQ07011
Gene ID (NCBI) 3604
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83893-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 9
Synonyms ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor, 4 1BB
GenBank accession numberBC006196
Gene symbol TNFRSF9/CD137
UniprotQ07011
Gene ID (NCBI) 3604
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.