Osteoprotegerin/TNFRSF11B Polyclonal antibody

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  WB analysis of human milk using 11534-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human milk using 11534-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 11534-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 11534-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11534-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Osteoprotegerin antibody (11534-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Osteoprotegerin/TNFRSF11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11534-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tumor necrosis factor receptor superfamily member 11B, TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG

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Tested Applications

• Positive WB detected in: human milk
• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11534-1-AP targets Osteoprotegerin/TNFRSF11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Osteoprotegerin/TNFRSF11B fusion protein Ag2139
• Full Name: tumor necrosis factor receptor superfamily, member 11b
• Calculated Molecular Weight: 401 aa, 46 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC030155
• Gene Symbol: TNFRSF11B
• Gene ID (NCBI): 4982
• RRID: AB_3085376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00300
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promoting bone formationis. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

Publications

Species	Application	Title
human	WB	Anal Cell Pathol (Amst); Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy; Authors - Pei-Long Liu
rat,human	IHC	BMC Oral Health; Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats; Authors - Jinghong Luo
	WB	Foods; Optimization of the Preparation Process and Ameliorative Efficacy in Osteoporotic Rats of Peptide-Calcium Chelates from Skipjack Tuna (Katsuwonus pelamis) Meat; Authors - Wan-Zhen Yan