Anti-Mouse TNFR2/CD120b Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98021-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TNFR2/CD120b Rabbit Recombinant Antibody (98021-1-RR, Clone: 240200D4) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98021-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98021-1-RR against Mouse Tnfrsf1b were performed. The affinity constant is 3.19 nM.

TNFR2/CD120b Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98021-1-RR

Clone No.240200D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD120b, TNFR2, Tnfrsf1b, 240200D4, TNF R2

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98021-1-RR targets TNFR2/CD120b in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: tumor necrosis factor receptor superfamily, member 1b
• Calculated Molecular Weight: 50KD
• GenBank Accession Number: NM_011610.3
• Gene Symbol: Tnfrsf1b
• Gene ID (NCBI): 21938
• RRID: AB_3672165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q545P4
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).