Anti-Human TNFR2/CD120b Rabbit Recombinant Antibody

TNFR2/CD120b Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230328B6

Cat no : 98010-2-RR

Synonyms

TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98010-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TNFR2/CD120b Rabbit Recombinant Antibody (98010-2-RR, Clone:230328B6) and PE-conjugated Goat Anti-Rabbit IgG, or 0.25 ug Isotype Control. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98010-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98010-2-RR against Human TNFR2/CD120b were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98010-2-RR targets TNFR2/CD120b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tumor necrosis factor receptor superfamily, member 1B
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC052977
• Gene Symbol: TNFR2
• Gene ID (NCBI): 7133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).