Anti-Human TNFR2/CD120b Rabbit Recombinant Antibody, PBS Only

TNFR2/CD120b Uni-rAb^TM Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230328B3

Cat no : 98010-1-PBS

Synonyms

TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept

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Validation Data Gallery

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  FC experiment of human PBMCs using 98010-1-RR (same clone as 98010-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TNFR2/CD120b (98010-1-RR, Clone:230328B3) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98010-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98010-1-PBS

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TNFR2/CD120b (98010-1-PBS, Clone:230328B3) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Cytometric bead array standard curve of MP00777-1

  Cytometric bead array standard curve of MP00777-1, TNFRSF1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83101-2-PBS. Detection antibody: 98010-1-PBS. Standard: Eg0086. Range: 0.625-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00777-2

  Cytometric bead array standard curve of MP00777-2, TNFRSF1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83101-3-PBS. Detection antibody: 98010-1-PBS. Standard: Eg0086. Range: 0.625-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00777-3

  Cytometric bead array standard curve of MP00777-3, TNFRSF1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83101-4-PBS. Detection antibody: 98010-1-PBS. Standard: Eg0086. Range: 0.625-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 98010-1-RR

  Surface Plasmon Resonance (SPR) kinetic assays of 98010-1-RR against Human TNFR2/CD120b were performed. The affinity constant is 7.52 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98010-1-PBS targets TNFR2/CD120b as part of a matched antibody pair:

MP00777-1: 83101-2-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)

MP00777-2: 83101-3-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)

MP00777-3: 83101-4-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tumor necrosis factor receptor superfamily, member 1B
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC052977
• Gene Symbol: TNFR2
• Gene ID (NCBI): 7133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).