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TNFR2 Recombinant antibody, PBS Only

TNFR2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230328B6

Cat no : 83101-1-PBS

Synonyms

TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFR2 Recombinant antibody (83101-1-RR)

    WB analysis using 83101-1-RR (same clone as 83101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83101-1-RR (TNFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83101-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83101-1-RR

    Affinity and Kinetic Characterization of 83101-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83101-1-RR against Human TNFR2 were performed. The affinity constant is 0.43 nM.

Product Information

83101-1-PBS targets TNFR2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name tumor necrosis factor receptor superfamily, member 1B
Calculated Molecular Weight 48 kDa
Observed Molecular Weight75 kDa, 65 kDa
GenBank Accession NumberBC052977
Gene Symbol TNFR2
Gene ID (NCBI) 7133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).