TNFR2 / TNFRSF1B Polyclonal antibody

TNFR2 / TNFRSF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28746-1-AP

Synonyms

CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

---

Validation Data Gallery

• 

  WB analysis using 28746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 28746-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TNFR2 / TNFRSF1B antibody (28746-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: THP-1 cells, U-937 cells
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

28746-1-AP targets TNFR2 / TNFRSF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFR2 / TNFRSF1B fusion protein Ag30490
• Full Name: tumor necrosis factor receptor superfamily, member 1B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 75 kDa, 65 kDa
• GenBank Accession Number: BC052977
• Gene Symbol: TNFR2
• Gene ID (NCBI): 7133
• RRID: AB_2881205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Publications

Species	Application	Title
human	WB	PLoS Pathog; SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure; Authors - Huilong Li
human	IF	Am J Pathol; TNF receptor-2 signals clear-cell renal carcinoma proliferation via phosphorylated-4EBP1 and mitochondrial gene translation; Authors - Rafia S Al-Lamki