TNFR1/CD120a Recombinant antibody

TNFR1/CD120a Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241336B4

Cat no : 84243-5-RR

Synonyms

CD120a, TNFR1, TNFRSF1A, 241336B4, p60

---

Validation Data Gallery

• 

  WB analysis using 84243-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 84243-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 84243-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84243-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84243-5-RR against Human TNFR1/CD120a were performed. The affinity constant is 20.5 nM.

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells, HL-60 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84243-5-RR targets TNFR1/CD120a in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor receptor superfamily, member 1A
• Calculated Molecular Weight: 455 aa, 50 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC010140
• Gene Symbol: TNFR1
• Gene ID (NCBI): 7132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.