TNFR1 Recombinant antibody, PBS Only (Capture)

TNFR1 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241337C1

Cat no : 84243-6-PBS

Synonyms

CD120a, TNFRSF1A, 241337C1, p60, p55 R

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00099-3

  Sandwich ELISA standard curve of MP00099-3, Human TNFR1/CD120a Recombinant Matched Antibody Pair - PBS only. 84243-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02331. 84243-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP00099-3

  Urine of six individual healthy human donors was measured. The human TNFR1/CD120a concentration of detected samples was determined to be 264.6 pg/mL with a range of 64.6 - 815.7 pg/mL.

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  Sample test of MP00099-3

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNFR1/CD120a concentration was determined to be 95.3 pg/mL in unstimulated PBMC supernatant, 156.9 pg/mL in PHA stimulated PBMC supernatant.

• 

  Sample test of MP00099-3

  Serum of six individual healthy human donors was measured. The human TNFR1/CD120a concentration of detected samples was determined to be 2,420.0 pg/mL with a range of 1,829.1 - 3,374.3 pg/mL.

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Product Information

84243-6-PBS targets TNFR1 as part of a matched antibody pair:

MP00099-3: 84243-6-PBS capture and 84243-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor receptor superfamily, member 1A
• Calculated Molecular Weight: 455 aa, 50 kDa
• GenBank Accession Number: BC010140
• Gene Symbol: TNFR1
• Gene ID (NCBI): 7132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.