TNFAIP8 Polyclonal antibody

• Featured Product
• KD/KO Validated

TNFAIP8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 15790-1-AP

Synonyms

GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8

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Validation Data Gallery

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  WB analysis using 15790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15790-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 15790-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: K-562 cells, PC-13 cells, A549 cells, MOLT-4 cells
• Positive IHC detected in: human lung cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

15790-1-AP targets TNFAIP8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFAIP8 fusion protein Ag8491
• Full Name: tumor necrosis factor, alpha-induced protein 8
• Calculated Molecular Weight: 198 aa, 23 kDa
• Observed Molecular Weight: 19-23 kDa
• GenBank Accession Number: BC007014
• Gene Symbol: TNFAIP8
• Gene ID (NCBI): 25816
• RRID: AB_10792805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested. There are four major isoforms of TNFAIP8.

Publications

Species	Application	Title
	WB	J Immunol; Crucial Roles of TNFAIP8 Protein in Regulating Apoptosis and Listeria Infection.; Authors - Thomas P Porturas
human	WB	Oncotarget; TNFAIP8 promotes prostate cancer cell survival by inducing autophagy.; Authors - Suryakant Niture ; KD Validated
human	WB	Toxins (Basel); Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis; Authors - Suryakant Niture
human	IHC	J Proteome Res; Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.; Authors - Jing Xiao ; KD Validated
mouse	IF	Sci Rep; The TIPE Molecular Pilot That Directs Lymphocyte Migration in Health and Inflammation.; Authors - Honghong Sun ; KO Validated
mouse	WB	Sci Rep; The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.; Authors - Ji-Soo Kim ; KD Validated