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  • KD/KO Validated

TSG-6 Polyclonal antibody

TSG-6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13321-1-AP

Synonyms

Hyaluronate binding protein, TNF alpha induced protein 6, TNF stimulated gene 6 protein, TNFAIP6, TSG 6, TSG6, TSG-6



Tested Applications

Positive WB detected inNIH/3T3 cells, A549 cells
Positive IHC detected inrat kidney tissue, human cervical cancer tissue, human ovary tumor tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13321-1-AP targets TSG-6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSG-6 fusion protein Ag3885
Full Name tumor necrosis factor, alpha-induced protein 6
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC030205
Gene Symbol TNFAIP6
Gene ID (NCBI) 7130
RRIDAB_2204641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TSG-6 antibody 13321-1-APDownload protocol
IHC protocol for TSG-6 antibody 13321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JACC Basic Transl Sci

Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6.

Authors - Rena Watanabe
mouseWB

Front Pharmacol

TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation.

Authors - Yue-Meng Wan
ratWB

Neurochem Res

Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage.

Authors - Bin Tang
humanWB,IHC

Contrast Media Mol Imaging

TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway

Authors - Hongquan Cui
  • KD Validated
humanWB,IHC,IF

Neural Regen Res

Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis

Authors - Mingxiang Ding
humanWB,IHC

Front Immunol

Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma

Authors - Renwang Liu