TNFAIP3 Polyclonal antibody

TNFAIP3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 23456-1-AP

Synonyms

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37

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Validation Data Gallery

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  WB analysis of Jurkat using 23456-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23456-1-AP (TNFAIP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23456-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23456-1-AP (TNFAIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23456-1-AP (TNFAIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

23456-1-AP targets TNFAIP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFAIP3 fusion protein Ag18150
• Full Name: tumor necrosis factor, alpha-induced protein 3
• Calculated Molecular Weight: 790 aa, 90 kDa
• Observed Molecular Weight: 80-90 kDa, 37-40 kDa
• GenBank Accession Number: BC114480
• Gene Symbol: TNFAIP3
• Gene ID (NCBI): 7128
• RRID: AB_2879284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Upon T- or B-cell receptor engagement human (h) A20 is cleaved by MALT1 after arginine 439, yielding an N-terminal fragment and a C-terminal one (~37-40 kDa). This antibody, which is a rabbit polyclonal antibody raised against 350 aa of C-terminus of human TNFAIP3, can recognize full-length A20 (~90 kDa) and cleaved form (~37-40 kDa).

Publications

Species	Application	Title
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human,mouse	WB,IHC	Am J Cancer Res; ZY-444 inhibits the growth and metastasis of prostate cancer by targeting TNFAIP3 through TNF signaling pathway; Authors - Meng-Ting Han
mouse	WB	Biomedicines; The Cholinergic Anti-Inflammatory Pathway Attenuates the Development of Atherosclerosis in Apoe-/- Mice through Modulating Macrophage Functions.; Authors - Zhengjiang Qian
		Biomedicines; Sertoli Cells Express Accommodation, Survival, and Immunoregulatory Factors When Exposed to Normal Human Serum; Authors - Rachel L Washburn
rat	WB	Int Immunopharmacol; TNFAIP3-upregulated RIP3 exacerbates acute pancreatitis via activating NLRP3 inflammasome.; Authors - Yifan Wang
human	WB	Mol Cell Biol; LncRNA HOXA-AS3 integrates NF-κB signaling to regulate endothelium inflammation.; Authors - Xinxing Zhu