TNFAIP1 Monoclonal antibody

TNFAIP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4F11B5

Cat no : 60327-1-Ig

Synonyms

B12, B61, BACURD2, EDP1, TNFAIP1

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Validation Data Gallery

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  WB analysis of HUVEC using 60327-1-Ig

  HUVEC, HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60327-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60327-1-Ig (TNFAIP1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60327-1-Ig targets TNFAIP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TNFAIP1 fusion protein Ag7873
• Full Name: tumor necrosis factor, alpha-induced protein 1 (endothelial)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001949
• Gene Symbol: TNFAIP1
• Gene ID (NCBI): 7126
• RRID: AB_2881437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncotarget; PMID: 29137317; Authors - Wu Shuang