TNFAIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TNFAIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15320-1-AP

Synonyms

Tumor necrosis factor, alpha-induced protein 1, endothelial, EDP1, BTB/POZ domain-containing protein TNFAIP1, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 15320-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15320-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15320-1-AP

  Immunofluorescent analysis of HeLa cells using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells
• Positive IHC detected in: human placenta tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

15320-1-AP targets TNFAIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFAIP1 fusion protein Ag0220
• Full Name: tumor necrosis factor, alpha-induced protein 1 (endothelial)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001949
• Gene Symbol: TNFAIP1
• Gene ID (NCBI): 7126
• RRID: AB_10644288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.

Publications

Species	Application	Title
mouse	WB	Neurotox Res; TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells.; Authors - Junzhi Yi ; KD Validated
human	WB	Oncol Lett; MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis.; Authors - Zhang Xiaoting X ; KD Validated
mouse	WB	Cell Commun Signal; Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination; Authors - Wei Wang