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TNF-alpha Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 3.9-250 pg/mL

Cat No : MP00702-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

    Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Serum of seven individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 3.25 pg/mL with a range of ND-9.1 pg/mL.

    Sample test of MP00702-4

    Serum of seven individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 3.25 pg/mL with a range of ND-9.1 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP00702-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00702-4 targets TNF-alpha in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 3.9-250 pg/mL (Sandwich ELISA);

Capture antibody: TNF-alpha Recombinant antibody, PBS Only (Capture) 83721-5-PBS (240668E6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: TNF-alpha Recombinant antibody, PBS Only (Detector) 83721-6-PBS (240668A4). 100 μg. Concentration 1 mgl/ml.

Alternative TNF-alpha matched antibody pairs: MP00682-1, MP00682-2, MP00682-3, MP00702-1, MP00702-2, MP00702-3, MP50093-1

Matched pair protein standard: Eg0816

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83721-5-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (TNF superfamily, member 2)
Synonyms TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1
GenBank accession numberNM_000594.4
Gene symbol TNF-alpha
UniprotP01375
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83721-6-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (TNF superfamily, member 2)
Synonyms TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1
GenBank accession numberNM_000594.4
Gene symbol TNF-alpha
UniprotP01375
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.