Tested Applications
| Positive WB detected in | THP-1 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26405-1-AP targets TNF-alpha in WB, IHC, IF/ICC, ELISA, Blocking assay applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, rabbit, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24020 Product name: Recombinant human TNF-a protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-233 aa of BC028148 Sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL Predict reactive species |
| Full Name | tumor necrosis factor (TNF superfamily, member 2) |
| Calculated Molecular Weight | 233 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC028148 |
| Gene Symbol | TNF-alpha |
| Gene ID (NCBI) | 7124 |
| ENSEMBL Gene ID | ENSG00000232810 |
| RRID | AB_2918102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01375 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TNF-alpha antibody 26405-1-AP | Download protocol |
| IHC protocol for TNF-alpha antibody 26405-1-AP | Download protocol |
| WB protocol for TNF-alpha antibody 26405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TNF-alpha Polyclonal antibody (Cat# 26405-1-AP) has 86 citations published in journals including ACS Nano, Redox Biology, Advanced Science, Science Advances, Journal of Controlled Release, Cell Death & Disease, Theranostics, Materials Today Bio, International Immunopharmacology, International Journal of Molecular Sciences, and Scientific Reports. Research fields span inflammation and immunology, neurodegeneration, wound healing, oncology, hepatic and renal disease, cardiovascular pathology, diabetes, sepsis, rheumatoid arthritis, and pharmacology of traditional Chinese medicine.
| Species | Application | Title |
|---|---|---|
ACS Nano Dual-Pronged Attack: Biomimetic Multi-Functional MOFs for Rheumatoid Arthritis Therapy via Inhibiting Synovial Hyperplasia and Improving Immune Microenvironment | ||
Redox Biol Ethyl carbamate triggers ferroptosis in liver through inhibiting GSH synthesis and suppressing Nrf2 activation. | ||
J Nanobiotechnology A single-dose of PDGFB circular RNA enables sustained growth factor expression to accelerate diabetic wound healing. | ||
J Nanobiotechnology Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors | ||
Theranostics A strain-programmed lignin-based Janus patch for rapid healing of postoperative rectal wounds | ||
Adv Sci (Weinh) Deciphering the Role and Mechanism of Decidual Monocyte-Derived Macrophage Infiltration in Obstetric Antiphospholipid Syndrome at Single-Cell Resolution. |
