TNF-alpha Polyclonal antibody

TNF-alpha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 26162-1-AP

Synonyms

DIF, Tnf, TNF alpha, Tnfa, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, TNFα, TNF-α, tumor necrosis factor

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Validation Data Gallery

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  WB analysis using 26162-1-AP

  Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26162-1-AP (Tnf alpha antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

26162-1-AP targets TNF-alpha in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNF-alpha fusion protein Ag24089
• Full Name: tumor necrosis factor
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM-013693
• Gene Symbol: TNF-alpha
• Gene ID (NCBI): 21926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice; Authors - Qiong Fu
mouse	WB	J Control Release; Microenvironment-responsive nano-bioconjugated vesicles for the multi-pronged treatment of liver fibrosis; Authors - Jie-Hua Xing
rat	IHC	ACS Appl Mater Interfaces; Evaluation of the Antibacterial, Anti-Inflammatory, And Bone-Promoting Capacity of UiO-66 Loaded with Thymol or Carvacrol; Authors - Minghe Zheng
mouse	IHC	Cell Mol Biol Lett; Single-cell view and a novel protective macrophage subset in perivascular adipose tissue in T2DM; Authors - Jiaxuan Li
mouse	WB	Biomed Pharmacother; Antinociceptive and neuroprotective effect of echinacoside on peripheral neuropathic pain in mice through inhibiting P2X7R/FKN/CX3CR1 pathway; Authors - Ning Liu
mouse	WB	Int J Med Sci; Glutamine Protects against Mouse Abdominal Aortic Aneurysm through Modulating VSMC Apoptosis and M1 Macrophage Activation; Authors - Jinxi Wang