Anti-Human TNF beta Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98056-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF beta Rabbit Recombinant Antibody (98056-1-RR, Clone: 240393C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98056-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98056-1-RR against Human TNF Beta were performed. The affinity constant is below 1 pM.

TNF beta Recombinant Antibody for FC (Intra)

Cat No. 98056-1-RR

Clone No.240393C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

LTA, TNF β, Lymphotoxin-alpha, Lymphotoxin alpha, LT alpha

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Tested Applications

• Positive FC (Intra) detected in: Anti-CD3/CD28 treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98056-1-RR targets TNF beta in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: lymphotoxin alpha (TNF superfamily, member 1)
• Calculated Molecular Weight: 205 aa, 22 kDa
• GenBank Accession Number: BC034729
• Gene Symbol: TNF beta
• Gene ID (NCBI): 4049
• RRID: AB_3672203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01374
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.