Anti-Human TNF Beta Rabbit Recombinant Antibody

TNF Beta Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240393C3

Cat no : 98056-1-RR

Synonyms

LTA, TNF β, Lymphotoxin-alpha, Lymphotoxin alpha, LT alpha

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Validation Data Gallery

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  FC experiment of human PBMCs using 98056-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNFB Rabbit Recombinant Antibody (98056-1-RR, Clone: 240393C3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98056-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98056-1-RR against Human TNF Beta were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC (Intra) detected in: Anti-CD3/CD28 treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98056-1-RR is TNF Beta Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: lymphotoxin alpha (TNF superfamily, member 1)
• Calculated Molecular Weight: 205 aa, 22 kDa
• GenBank Accession Number: BC034729
• Gene Symbol: TNF beta
• Gene ID (NCBI): 4049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.