TNF beta Polyclonal antibody

TNF beta Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31505-1-AP

Synonyms

LTA, LT alpha, TNFB, TNF β, Lymphotoxin-alpha

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Validation Data Gallery

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  WB analysis using 31505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 31505-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 31505-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31505-1-AP targets TNF beta in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lymphotoxin alpha (TNF superfamily, member 1)
• Calculated Molecular Weight: 205 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC034729
• Gene Symbol: TNF beta
• Gene ID (NCBI): 4049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.