- Featured Product
- KD/KO Validated
TMPRSS4 Polyclonal antibody
TMPRSS4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11283-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SW 1990 cells, BxPC-3 cells, mouse stomach tissue |
| Positive IHC detected in | human pancreas cancer tissue, human colon tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 13 publications below |
| IF | See 4 publications below |
Product Information
11283-1-AP targets TMPRSS4 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMPRSS4 fusion protein Ag1804 |
| Full Name | transmembrane protease, serine 4 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC011703 |
| Gene Symbol | TMPRSS4 |
| Gene ID (NCBI) | 56649 |
| RRID | AB_2203508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMPRSS4, also named as CAPH2 and MT-SP2, belongs to the peptidase S1 family. TMPRSS4 is a novel type II transmembrane serine protease that is highly expressed on the cell surface in pancreatic, thyroid and other cancer tissues. It promotes invasion, migration and metastasis of human tumor cells by facilitating an epithelial-mesenchymal transition(EMT)(PMID:20118200).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMPRSS4 antibody 11283-1-AP | Download protocol |
| IHC protocol for TMPRSS4 antibody 11283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Angiotensin-converting Enzyme 2-containing Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein. | ||
Cancer Res TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation. | ||
Int J Mol Sci Cardiac and Renal SARS-CoV-2 Viral Entry Protein Regulation by Androgens and Diet: Implications for Polycystic Ovary Syndrome and COVID-19. | ||
Carcinogenesis TMPRSS4 induces invasion and epithelial-mesenchymal transition through upregulation of integrin alpha5 and its signaling pathways. | ||
Cancer Lett TMPRSS4 induces cancer stem cell-like properties in lung cancer cells and correlates with ALDH expression in NSCLC patients. | ||
Front Immunol TSPAN1, TMPRSS4, SDR16C5, and CTSE as Novel Panel for Pancreatic Cancer: A Bioinformatics Analysis and Experiments Validation. |
