TMOD4 Polyclonal antibody, PBS Only

TMOD4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11753-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 11753-1-AP (same clone as 11753-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11753-1-AP (same clone as 11753-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11753-1-AP (same clone as 11753-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse skeletal muscle using 11753-1-AP (same clone as 11753-1-PBS)

  IP result of anti-TMOD4 (IP:11753-1-AP, 4ug; Detection:11753-1-AP 1:600) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11753-1-AP (same clone as 11753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11753-1-AP (TMOD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

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Product Information

11753-1-PBS targets TMOD4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMOD4 fusion protein Ag2349
• Full Name: tropomodulin 4 (muscle)
• Calculated Molecular Weight: 345 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC017810
• Gene Symbol: TMOD4
• Gene ID (NCBI): 29765
• RRID: AB_2205433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.