TMIGD1 Polyclonal antibody, PBS Only

TMIGD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27174-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

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Product Information

27174-1-PBS targets TMIGD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMIGD1 fusion protein Ag25691
• Full Name: transmembrane and immunoglobulin domain containing 1
• Calculated Molecular Weight: 262 aa, 29 kDa
• Observed Molecular Weight: 45 kDa, 29 kDa
• GenBank Accession Number: BC137201
• Gene Symbol: TMIGD1
• Gene ID (NCBI): 388364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).