TMEM74 Polyclonal antibody

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  IHC staining of mouse brain using 25506-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25506-1-AP (TMEM74 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM74 Polyclonal Antibody for IHC, ELISA

Cat No. 25506-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Transmembrane protein 74, NET36

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25506-1-AP targets TMEM74 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM74 fusion protein Ag22288
• Full Name: transmembrane protein 74
• Calculated Molecular Weight: 305 aa, 33 kDa
• GenBank Accession Number: BC030710
• Gene Symbol: TMEM74
• Gene ID (NCBI): 157753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96NL1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been reported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)