TMEM65 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TMEM65 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21913-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21913-1-AP (same clone as 21913-1-PBS)

  WB result of TMEM65 antibody (21913-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HeLa cells. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 21913-1-AP (same clone as 21913-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Huh-7 using 21913-1-AP (same clone as 21913-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Huh-7 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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Product Information

21913-1-PBS targets TMEM65 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM65 fusion protein Ag15509
• Full Name: transmembrane protein 65
• Calculated Molecular Weight: 240 aa, 26 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC032396
• Gene Symbol: TMEM65
• Gene ID (NCBI): 157378
• RRID: AB_2918075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.