TMEM65 antibody (21913-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 21913-1-AP (same clone as 21913-1-PBS)
WB result of TMEM65 antibody (21913-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HEK-293 cells. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.
WB analysis using 21913-1-AP (same clone as 21913-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.
WB analysis using 21913-1-AP (same clone as 21913-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 21913-1-AP (same clone as 21913-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.
IF Staining of Huh-7 using 21913-1-AP (same clone as 21913-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Huh-7 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

21913-1-PBS targets TMEM65 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMEM65 fusion protein Ag15509 Predict reactive species
Full Name	transmembrane protein 65
Calculated Molecular Weight	240 aa, 26 kDa
Observed Molecular Weight	20-25 kDa
GenBank Accession Number	BC032396
Gene Symbol	TMEM65
Gene ID (NCBI)	157378
RRID	AB_2918075
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6PI78
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.