TMEM41B Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM41B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 29270-1-AP

Synonyms

Transmembrane protein 41B, KIAA0033

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Validation Data Gallery

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  WB analysis of HeLa using 29270-1-AP

  WB result of TMEM41B antibody (29270-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM41B transfected HeLa cells.

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  WB analysis using 29270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29270-1-AP (TMEM41B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: mouse lung tissue, mouse testis tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

29270-1-AP targets TMEM41B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM41B fusion protein Ag30810
• Full Name: transmembrane protein 41B
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 22-28 kDa
• GenBank Accession Number: BC035034
• Gene Symbol: TMEM41B
• Gene ID (NCBI): 440026
• RRID: AB_2918264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.

Publications

Species	Application	Title
mouse	WB,IF	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang ; KO Validated
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB	STAR Protoc; In vitro and in vivo assay of the ER lipid scramblase TMEM41B.; Authors - Lingzhi Wu ; KO Validated
mouse,pig	WB,IF	J Virol; TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication; Authors - Xiu-Qing Li ; KD Validated
human	WB	Nature; PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection; Authors - Dijin Xu