Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, mouse lung tissue, mouse testis tissue|
|Positive IHC detected in||mouse lung tissue, mouse testis tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
29270-1-AP targets TMEM41B in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TMEM41B fusion protein Ag30810|
|Full Name||transmembrane protein 41B|
|Observed molecular weight||22-28 kDa|
|GenBank accession number||BC035034|
|Gene ID (NCBI)||440026|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.
TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.
Comparative Proteomic Analysis of tPVAT during Ang II Infusion.
In vitro and in vivo assay of the ER lipid scramblase TMEM41B.
TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection