TMEM27 Recombinant antibody

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  WB analysis using 82853-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82853-2-RR (TMEM27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82853-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82853-2-RR against Human TMEM27 were performed. The affinity constant is below 1 pM.

TMEM27 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 82853-2-RR

Clone No.240437C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NX17, NX 17, Collectrin, CLTRN, ACE2

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82853-2-RR targets TMEM27 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM27 fusion protein Ag7668
• Full Name: transmembrane protein 27
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC050606
• Gene Symbol: TMEM27
• Gene ID (NCBI): 57393
• RRID: AB_3670578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9HBJ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated ins secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).