TMEM200C Polyclonal antibody

TMEM200C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28596-1-AP

Synonyms

TTMA, Transmembrane protein TTMA, Transmembrane protein 200C

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Validation Data Gallery

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  WB analysis using 28596-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28596-1-AP (TMEM200C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 28596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 28596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28596-1-AP targets TMEM200C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM200C fusion protein Ag29577
• Full Name: two transmembrane domain family member A
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_001080209
• Gene Symbol: TTMA
• Gene ID (NCBI): 645369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM200C (Transmembrane protein 200C) may be an integral component of the membrane.