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TMEM175 Recombinant antibody, PBS Only

TMEM175 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241567B9

Cat no : 84554-1-PBS

Synonyms

Transmembrane protein 175, hTMEM175, 241567B9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM175 Recombinant antibody (84554-1-RR)

    WB analysis using 84554-1-RR (same clone as 84554-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84554-1-RR (TMEM175 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84554-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84554-1-RR

    Affinity and Kinetic Characterization of 84554-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84554-1-RR against Human TMEM175 were performed. The affinity constant is 5.17 nM.

Product Information

The immunogen of 84554-1-PBS is TMEM175 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM175 fusion protein Ag13890
Full Name transmembrane protein 175
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight41-45 kDa, 54-60 kDa
GenBank Accession NumberBC005158
Gene Symbol TMEM175
Gene ID (NCBI) 84286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.