Product Information
84554-1-PBS targets TMEM175 in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TMEM175 fusion protein Ag13890 Predict reactive species |
| Full Name | transmembrane protein 175 |
| Calculated Molecular Weight | 504 aa, 56 kDa |
| Observed Molecular Weight | 41-45 kDa, 54-60 kDa |
| GenBank Accession Number | BC005158 |
| Gene Symbol | TMEM175 |
| Gene ID (NCBI) | 84286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9BSA9 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.
