TMEM175 Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM175 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 19925-1-AP

Synonyms

Transmembrane protein 175, hTMEM175

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Validation Data Gallery

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  WB analysis using 19925-1-AP

  Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, SH-SY5Y cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

19925-1-AP targets TMEM175 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM175 fusion protein Ag13890
• Full Name: transmembrane protein 175
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 41-45 kDa, 54-60 kDa
• GenBank Accession Number: BC005158
• Gene Symbol: TMEM175
• Gene ID (NCBI): 84286
• RRID: AB_10666166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

Publications

Species	Application	Title
	WB	Nature; A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.; Authors - Jinhong Wie ; KO Validated
human	WB	Cell; TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function.; Authors - Chunlei Cang
human	WB	Ann Neurol; Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies.; Authors - Lynne Krohn
human	WB	Proc Natl Acad Sci U S A; TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.; Authors - Sarah Jinn ; KO Validated
mouse	WB	Mol Med; Modulation of anti-cardiac fibrosis immune responses by changing M2 macrophages into M1 macrophages; Authors - Shiqi Chen
rat	WB	Mol Brain; TMEM175 mediates Lysosomal function and participates in neuronal injury induced by cerebral ischemia-reperfusion.; Authors - Mengling Zhang