TMEM132A Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM132A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25301-1-AP

Synonyms

GBP, HSPA5 binding protein 1, HSPA5BP1, KIAA1583, TMEM132A, transmembrane protein 132A

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Validation Data Gallery

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  WB analysis using 25301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25301-1-AP (TMEM132A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 25301-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25301-1-AP (TMEM132A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25301-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25301-1-AP (TMEM132A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HaCaT cells, fetal human brain tissue, MCF-7 cells, SH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

25301-1-AP targets TMEM132A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM132A fusion protein Ag18142
• Full Name: transmembrane protein 132A
• Calculated Molecular Weight: 1023 aa, 110 kDa
• Observed Molecular Weight: 100-140 kDa
• GenBank Accession Number: BC114569
• Gene Symbol: TMEM132A
• Gene ID (NCBI): 54972
• RRID: AB_2880018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Front Cell Dev Biol; TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.; Authors - Binbin Li ; KO Validated
human,mouse	WB	Development; TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration; Authors - Binbin Li ; KO Validated