TMEM126A Polyclonal antibody

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  WB analysis using 20759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20759-1-AP (TMEM126A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 20759-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (20759-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

TMEM126A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20759-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TMEM126, TMEM, OPA7

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20759-1-AP targets TMEM126A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM126A fusion protein Ag14705
• Full Name: transmembrane protein 126A
• Calculated Molecular Weight: 195 aa, 22 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC007875
• Gene Symbol: TMEM126A
• Gene ID (NCBI): 84233
• RRID: AB_3669345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H061
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.