TMP21 Monoclonal antibody, PBS Only

TMP21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B11F9

Cat no : 67876-1-PBS

Synonyms

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

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Validation Data Gallery

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  WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMP21 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug TMP21 Monoclonal antibody (67876-1-Ig, Clone:3B11F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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Product Information

67876-1-PBS targets TMP21 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMP21 fusion protein Ag7516
• Full Name: transmembrane emp24-like trafficking protein 10 (yeast)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001825
• Gene Symbol: TMED10
• Gene ID (NCBI): 10972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.