TMED10 Monoclonal antibody
TMED10 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Pig, Mouse, Rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3B11F9
Cat no : 67876-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, pig liver tissue, HeLa cells, HepG2 cells, Jurkat cells, Raji cells, rat liver tissue, mouse liver tissue |
Positive IHC detected in | human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67876-1-Ig targets TMED10 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.
Tested Reactivity | Human, Pig, Mouse, Rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TMED10 fusion protein Ag7516 |
Full Name | transmembrane emp24-like trafficking protein 10 (yeast) |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC001825 |
Gene Symbol | TMED10 |
Gene ID (NCBI) | 10972 |
RRID | AB_2918633 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for TMED10 antibody 67876-1-Ig | Download protocol |
IHC protocol for TMED10 antibody 67876-1-Ig | Download protocol |
IF protocol for TMED10 antibody 67876-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |