TMBIM6 Recombinant antibody

TMBIM6 Uni-rAb^TM Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241589D12

Cat no : 84499-1-RR

Synonyms

TEGT, BI-1, BI1, BI 1, Bax inhibitor 1

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Validation Data Gallery

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  IHC staining of mouse liver using 84499-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84499-1-RR (TMBIM6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84499-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84499-1-RR (TMBIM6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 84499-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using TMBIM6 antibody (84499-1-RR, Clone: 241589D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84499-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84499-1-RR against Human TMBIM6 were performed. The affinity constant is 6.46 nM.

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Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84499-1-RR is TMBIM6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMBIM6 fusion protein Ag24780
• Full Name: transmembrane BAX inhibitor motif containing 6
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: BC000916
• Gene Symbol: TMBIM6
• Gene ID (NCBI): 7009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.