TLR8 Monoclonal antibody, PBS Only

TLR8 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B6A4

Cat no : 67317-1-PBS

Synonyms

CD288, TLR8, toll like receptor 8

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Validation Data Gallery

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  WB analysis using 67317-1-Ig (same clone as 67317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 67317-1-Ig (same clone as 67317-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67317-1-PBS targets TLR8 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLR8 fusion protein Ag16566
• Full Name: toll-like receptor 8
• Calculated Molecular Weight: 1041 aa, 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC101075
• Gene Symbol: TLR8
• Gene ID (NCBI): 51311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.