TLR5 Polyclonal antibody
TLR5 Polyclonal Antibody for IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 19810-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human small intestine tissue, human tonsillitis tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
19810-1-AP targets TLR5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TLR5 fusion protein Ag13840 |
| Full Name | toll-like receptor 5 |
| Calculated Molecular Weight | 858 aa, 98 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC109119 |
| Gene Symbol | TLR5 |
| Gene ID (NCBI) | 7100 |
| RRID | AB_10699876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TLR5 antibody 19810-1-AP | Download protocol |
| IP protocol for TLR5 antibody 19810-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway. | ||
Int J Mol Sci Myeloid-Derived Suppressor Cells Gain Suppressive Function during Neonatal Bacterial Sepsis. | ||
Int J Mol Sci Cross-Talk of Toll-Like Receptor 5 and Mu-Opioid Receptor Attenuates Chronic Constriction Injury-Induced Mechanical Hyperalgesia through a Protein Kinase C Alpha-Dependent Signaling. | ||
EBioMedicine Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease | ||
Oncotarget Toll-like receptor 5 gene polymorphism is associated with breast cancer susceptibility. | ||
Cells Fibronectin Functions as a Selective Agonist for Distinct Toll-like Receptors in Triple-Negative Breast Cancer. |
