Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

230463C7

Cat no : 98018-1-RR

Synonyms

Tlr4, CD284, toll like receptor 4, 230463C7

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Validation Data Gallery

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  FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Balb/c mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98018-1-RR is TLR4/CD284 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TLR4/CD284 fusion protein Ag32998
• Full Name: toll-like receptor 4
• Calculated Molecular Weight: 96 kDa
• GenBank Accession Number: NM_021297
• Gene Symbol: Tlr4
• Gene ID (NCBI): 21898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.