CoraLite® Plus 750 Anti-Human TLR3/CD283 Rabbit Recombinant Antibody

TLR3/CD283 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230445G9

Cat no : CL750-98016-2

Synonyms

TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445G9

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Validation Data Gallery

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  FC experiment of human monocyte-derived immature dendritic cells using CL750-98016-2

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 5 ul CoraLite® Plus 750 Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (CL750-98016-2, Clone: 230445G9) (right), or Isotype Control (left). Cells were co-stained with CoraLite® Plus 488 Anti-Human CD11c. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human monocyte-derived immature dendritic cells using CL750-98016-2

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 5 ul CoraLite® Plus 750 Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (CL750-98016-2, Clone: 230445G9) (red), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: human monocyte-derived immature dendritic cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-98016-2 is TLR3/CD283 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TLR3/CD283 fusion protein Ag34044
• Full Name: toll-like receptor 3
• Calculated Molecular Weight: 904 aa, 104 kDa
• GenBank Accession Number: BC094737
• Gene Symbol: TLR3
• Gene ID (NCBI): 7098
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).