Talin-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Talin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 14168-1-AP

Synonyms

TLN1, TLN, talin1, talin 1, KIAA1027

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Validation Data Gallery

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  WB analysis using 14168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14168-1-AP

  IP result of anti-Talin-1 (IP:14168-1-AP, 4ug; Detection:14168-1-AP 1:40000) with HeLa cells lysate 1320 ug.

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  IHC staining of human colon using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 14168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of A549 using 14168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin).

• 

  IF Staining of HepG2 using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14168-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Polyclonal antibody (14168-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, NIH/3T3 cells, C6 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, HeLa cells, HepG2 cells, A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Product Information

14168-1-AP targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Talin-1 fusion protein Ag5367
• Full Name: talin 1
• Calculated Molecular Weight: 2541 aa, 270 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: BC042923
• Gene Symbol: Talin-1
• Gene ID (NCBI): 7094
• RRID: AB_2271861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Publications

Species	Application	Title
human	IF	ACS Nano; Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.; Authors - Xiao Huang
human	WB,IHC,IF,IP	Elife; Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.; Authors - Yixiao Zhang ; KD Validated
human	IF	Mol Oncol; ITIH5 as a multifaceted player in pancreatic cancer suppression, impairing tyrosine kinase signaling, cell adhesion and migration; Authors - Jennifer Kosinski
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang ; KD Validated
mouse	IF	Mol Neurobiol; Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.; Authors - Qingxiu Yao
rat	WB	Front Pharmacol; Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway.; Authors - Lei Liu

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